Traditionally grand ducal Tuscany and its cultural politics have been viewed through the lens of absolutism. Based on a wide range of newly found sources and building on recent revisionist scholarship, this study uses the universities of Pisa and Siena to expose the contradictions and the tensions which characterised the grand duchy. Setting the universities against the diplomatic, military, administrative, economic, ecclesiastical, and cultural development of the grand duchy, it shows how innovation mixed with tradition and local privileges were not only upheld but extended significantly.