This new edition explores lab protocols describing new techniques to study cytotoxic T-cells (CTLs), as well as chapters of a more general discursive nature, all with an emphasis on the use of systems biology in immunology. Beginning with phenotypical characterization of CTL populations, the volume continues within vitroandin vivocytotoxicity assays, methods to detect senescent T cells,in vivoandin vitromodels to understand immune and bone cells cross-talk, microscopy andin vivoimaging, as well as "Omics" approaches and molecular methods, concluding with chapters on CTL involvement in transplantation and link microbiota-immunity. Written for the highly successfulMethods in Molecular Biologyseries, chapters feature the kind of detail and key implementation advice for best results in the lab. 

Authoritative and up-to-date,Cytotoxic T-Cells: Methods and Protocols, Second Editionserves as an ideal guidefor researchers working with these vital cells.