Excerpt from Decay and Soil Toxins: A Dissertation Submitted to the Faculty of the Ogden Graduate School of Science in Candidacy for the Degree of Doctor of Philosophy (Department of Botany)

More or less success has been attained by various workers in attempts to grow seed plants under sterile conditions. Harrison and barlow (11) tried sterilization by dry heat, moist heat, sulphuric acid, calcium hydrate, formaldehyde, and mercuric chloride, and abandoned all Of these means. They succeeded in getting sterile cultures of certain legumes by treating the unopened pods with mercuric chloride, opening them with flamed forceps, and transferring the seeds to a very small quantity of boiling water in sterile test tubes.

Wilson and harding (30) tried alcohol, formaldehyde, and mercuric chloride as a means of sterilizing alfalfa seeds, but found that when the seeds were sterile, the germination was very low.

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